The soft-clipped sequences that overhang the breakpoint are collapsed right into a consensus sequence using a several sequence alignment (MSA) presented during the seqAn library [27]. Wham applies a few filters to your consensus sequences. Breakpoints are certainly not described in circumstances in which consensus sequences are shorter than ten bp
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Wham clusters many of the candidate breakpoints and rounds their positions to the closest tenth foundation pair. The place with the best go through support is reported. If your comfortable-clipped consensus sequence may be aligned to your putative breakpoint area using the Smith-Waterman algorithm, the breakpoint is even further refined to the loca